《植物生理学报》 2013, 49(12): 1393-1399

干旱胁迫下CO₂浓度升高对转StAPX番茄植株耐旱能力的影响

孙卫红^1,*, 孙禛禛¹, 吴秋霞¹, 刘汉玄², 陈梅¹, 陈喆¹

江苏大学¹食品与生物工程学院, ²农业工程学院, 江苏镇江212013

通信作者：孙卫红；E-mail: weihongsun2009@163.com；Tel: 0511-88797059

摘　要：

在干旱胁迫伴随大气CO₂浓度以及升高的CO₂浓度(加倍)条件下, 以过量表达番茄类囊体膜抗坏血酸过氧化物酶基因(StAPX)的转基因番茄为试材, 探明干旱胁迫下CO₂浓度升高对转基因及其野生型番茄植株清除活性氧及耐旱能力的影响。结果表明: 升高的CO₂浓度明显增加了干旱胁迫下植物的光合水平; 升高的CO₂浓度明显降低了干旱导致的植物体内H₂O₂和O₂·ˉ的积累, 影响了干旱胁迫下番茄植株的水-水循环系统的活性氧清除酶活性和小分子抗氧化物质含量; 干旱胁迫下即使伴随升高的CO₂浓度, 测试番茄植株体内的渗透调节物质含量变化也不太明显; 升高的CO₂浓度明显降低了干旱胁迫下的植物细胞膜伤害程度; 干旱胁迫下, 升高的CO₂浓度对转基因番茄株系比对野生型植株的影响更加明显。结果证明干旱逆境下, 升高的CO₂浓度能够在一定程度上进一步提高转基因番茄植株的耐旱性。

关键词：干旱胁迫; 升高的CO₂浓度; 转基因番茄植株; 耐旱能力

收稿：2013-08-12   修定：2013-09-10

资助：江苏省自然科学基金面上项目(BK2010344)、作物生物学国家重点实验室开放基金(2011KF11)、江苏省博士后基金(1102132C)和国家博士后基金(2011M500867) 致谢 山东农业大学生命科学学院孟庆伟教授对本研究工作大力支持。

Effects of Elevated CO₂ Concentration on Drought Tolerance in Transgenic Tomato Plants under Drought Stress

SUN Wei-Hong^1,*, SUN Zhen-Zhen¹, WU Qiu-Xia¹, LIU Han-Xuan², CHEN Mei¹, CHEN Zhe¹

¹College of Food and Biological Engineering, ²Institute of Agricultural Engineering, Jiangsu University, Zhenjiang, Jiangsu 212013, China

Corresponding author: SUN Wei-Hong; E-mail: weihongsun2009@163.com; Tel: 0511-88797059

Abstract:

In this study, transgenic tomato plants over-expression of StAPX were tested under drought stress, companied with atmosphere and elevated (doubled) CO₂ concentration. The influences of elevated CO₂ concentration on the ROS-scavenging ability and the drought tolerance in transgenic and wild tomato plants under drought stress were studied. It was found that elevated CO₂ concentration increased the photosynthetic level in plants under drought stresses. Elevated CO₂ concentration reduced the accumulation of H₂O₂ and O₂·ˉ, and influenced antioxidant enzyme activities of water-water cycle system and small molecule antioxidant substance contents in tomato plants under drought stress conditions. Osmotic adjustment substances contents in tested plants changed little even at elevated CO₂ concentration under drought stresses. Elevated CO₂ concentration reduced the damage of cell membrane under drought stresses. The influence degree of elevated CO₂ concentration on transgenic lines was more outstanding than that in wild tomato plants. The results showed that elevated CO₂ concentration can increase the tolerance of transgenic tomato plants to drought stress to some extent.

Key words: drought stress; elevated CO₂ concentration; transgenic tomato plants; drought tolerance